PhD (2007) University of Connecticut
Norm is interested broadly in plant diversity and uses comparative genomic and phylogenomic approaches to understand the relationship between the diversification of genes, genomes, and species in plant groups ranging from mosses to angiosperms.
MS (2013) Northwestern University
Colby is interested in the evolution of meiosis and related genes in pre- and post-polyploid plant populations, with a current focus on the evolutionary history of meiosis-related genes and patterns of conservation and change across the phylogeny of green plants.
PhD (2017) Oklahoma State University
Angela is a plant systematist and evolutionary biologist who has worked with Asclepias and Solanum and is now leading the efforts to assemble, annotate, and analyze genomes of Schiedea. Angela is also providing expertise on many target-enrichment projects in the lab.
BA (2017) Brown University
Haley is interested in the evolution of plant volatiles and their role in plant-pollinator interactions. Currently, Haley studying the genetic basis of a floral scent polymorphism in Oenothera harringtonii using genome-wide association studies.
BA (2017) Oberlin College
Elena is interested in the intersection of phylogenetics and plant conservation, and is currently working on using allele histories to infer relationships in Schiedea.
BA (2020) University of Georgia
Juan was an REU student in 2019 and continues to work on pleurocarpous moss phylogenomics using over 800 genes. Juan is currently a PhD student at NYBG.
Please contact Norm if you are interested in graduate or undergraduate opportunities in the lab! Click HERE for more information!
Genome sequencing, phylogenomics, and HGT in diatoms
Chemical Ecology of plant defenses in Onagraceae
Pleurocarpous moss phylogenomics and transcriptomics
Phylo- and population genomics in Onagraceae
Analysis of performance of ortholog circumscription methods
Transcriptomes and historical selection in breadfruit
Leafy liverwort systematics (Acrobolbaceae)
Gene expression and a floral scent polymorphism
HybSeq and phylogenomics in Oenothera section Pachylophus
Protein conservation across diatom evolution
Intron conservation among multiple diatom genomes
Population genetics using RAD-Seq in Oenothera
Buxbaumia aphylla, a moss with a massive sporophyte and highly reduced gametophyte.
Unlocking the evolutionary history of Schiedea (Caryophyllaceae): rapid radiation of an endemic plant genus in the Hawaiian Islands
The primary aim of this project is to reconstruct the pattern of breeding system evolution in Schiedea through an integrated program of field, laboratory, and genomic studies. Nuclear genome sequencing, targeted sequence capture, GBS will be used to reconstruct a highly resolved phylogenetic tree of Schiedea, identify sources of phylogenetic conflict, and investigate patterns of historical introgression. The phylogenetic hypotheses developed will be used to interpret the evolution of breeding systems including transitions from hermaphroditism to dioecy, shifts from biotic to abiotic pollination, and traits associated with pollination biology (including scent and nectar production).
(Photo: Norm Wickett)
Collaborators: Ann Sakai & Steve Weller (UC Irvine), Mike Moore (Oberlin), Warren Wagner (NTBG, Smithsonian), Krissa Skogen (CBG), Lauren Weisenberger (USFWS)
Dimensions: Landscapes of Linalool – Scent mediated diversification of flowers and moths across Western North America
Plant scents are important drivers of the relationships between angiosperms and insects (herbivory, plant defense, pollination), but remain poorly integrated into our understanding of floral evolution and pollination ecology. This study examines the role of floral scent in the diversification of the western North American evening primroses (Onagraceae) and their pollinators (hawkmoths, bees) and floral and seed predators (Mompha moths). The research examines functional trait variation and selective forces in the field and experimental arrays, the genetic basis of the variation from the population to phylogenetic level using comparative genomics, and patterns of phylogenetic diversity in Onagraceae and Mompha.
(Photo: Krissa Skogen)
Collaborators: Krissa Skogen (Lead PI) & Jeremie Fant (CBG), Rachel Levin (Amherst), Rob Raguso (Cornell), Mike Moore (Oberlin), Warren Wagner (Smithsonian)
Evaluating the contributions of horizontally transferred bacterial genes and endogenous duplication events to the diversification of diatoms
The goal of this project is to identify the sources of novel diatom traits, which have contributed to their extraordinary metabolic and species-level diversity. Some traits are encoded by genes acquired from distantly related bacteria, an entirely different kingdom in the tree of life. Other genes appear to have been generated by processes acting within diatom genomes. This project will generate genome-scale data for 250 phylogenetically disparate diatom species. These data will be used to: (1) infer evolutionary relationships, (2) identify the sources - whether intrinsic or extrinsic - of new genes, and (3) correlate the pattern and timing of gene acquisitions with the origins of novel traits.
(Photo: Andy Alverson)
Collaborators: Andy Alverson (University of Arkansas), Matt Parks (Central Oklahoma University)
Assembling the Pleurocarpous Moss Tree of Life – Resolving the rapid radiation using genomics and transcriptomics
The moss lineage Hypnanae underwent an explosive radiation following its origin 165 mya. Today, it comprises about 5,000 species, but their grouping into evolutionary units is obscure. Inferences from extensive sets of genes sampled using target enrichment and transcriptomes will provide a robust framework to further our understanding of this diverse, and ecologically important lineage. This study focuses on untangling the relationships among species, on identifying the geologic or climatic shifts that triggered these events, and on understanding the significance of morphological innovations allowing adaptations to diverse habitats.
(Photo: Blanka Shaw)
Collaborators: Bernard Goffinet (UConn), Jon Shaw (Duke), Matt Johnson (Texas Tech), Rafa Medina (Augustana, Illinois), Yang Liu (Fairy Lake Botanical Garden, Shenzhen)
The Beagle Channel. Photo taken from the Chilean island of Navarino, home of the Omora Ethnobotanical Park.
Bechen, LL, MG Johnson, GT Broadhead, RA Levin, RP Overson, T Jogesh, JB Fant, RA Raguso, KA Skogen, NJ Wickett. 2021. Differential gene expression associated with a floral scent polymorphism in the evening primrose Oenothera harringtonii (Onagraceae). bioRxiv PREPRINT.
Ribeiro, CL, D Conde, KM Balmant, C Dervinis, MG Johnson, AP McGrath, P Szewczyk, F Unda, CA Finegan, HW Schmidt, B Miles, DR Drost, E Novaes, CA Gonzalez-Benecke, GF Peter, JG Burleigh, TA Martin, SD Mansfied, G Chang, NJ Wickett, M Kirst. 2020. The uncharacterized gene EVE contributes to vessel element dimensions in Populus. PNAS 117(9):5059-5066.
Wong, GK, DE Soltis, JH Leebens-Mack, NJ Wickett, MS Barker, Y Van de Peer, SW Graham, M Melkonian. 2020. Sequencing and analyzing the transcriptomes of a thousand species across the tree of life for green plants. Annual Review of Plant Biology 71:741-765.
Gardner, EM, MG Johnson, JT Pereira, ASA Puad, Sahromi, D Arifiani, NJ Wickett, NJC Zerega. 2020. Paralogs and off-target sequences improve phylogenetic resolution in a densely- sampled study of the breadfruit genus (Artocarpus, Moraceae). In press, Systematic Biology.
Carpenter EJ, N Matasci, S Ayyampalayam, S Wu, J Sun, J Yu, F Rocha J Vieira, C Bowler, RG Dorrell, MA Gitzendanner, L Li, W Du, KK Ullrich, NJ Wickett, TJ Barkmann, MS Barker, JH Leebens-Mack, GKS Wong. 2019. Access to RNA-sequencing data from 1,173 plant species: The 1000 Plant transcriptomes initiative (1KP). GigaScience 8(10):giz126. OPEN ACCESS
Medina, R, MG Johnson, Y Liu, NJ Wickett, AJ Shaw, B Goffinet. 2019. Phylogenomic delineation of Physcomitrium (Bryophyta: Funariaceae) based on targeted sequencing of nuclear exons and their flanking regions rejects the retention of Physcomitrella, Physcomitridium and Aphanorrhegma. Journal of Systematics and Evolution 57(4):404-417. FREE ACCESS
Johnson, MG, L Pokorny, S Dodsworth, LR Botigué, RS Cowan, A Devault, WL Eiserhardt, N Epitawalage, F Forest, JT Kim, JH Leebens-Mack, IJ Leitch, O Maurin, DE Soltis, PS Soltis, GKS Wong, WJ Baker, NJ Wickett. 2019. A Universal Probe Set for Targeted Sequencing of 353 Nuclear Genes from Any Flowering Plant Designed Using k-medoids Clustering. Systematic Biology 68(4): 594-606. OPEN ACCESS
Bruzzese, DJ, DL Wagner, T Harrison, T Jogesh, RP Overson, NJ Wickett, RA Raguso, KA Skogen. 2019. Phylogeny, host use, and diversification in the moth family Momphidae (Lepidoptera: Gelechioidea). PLoS ONE 14(6): e0207833. OPEN ACCESS
Liu, Y, MG Johnson, CJ Cox, R Medina, N Devos, A Vanderpoorten, L Hedenäs, NE Bell, JR Shevock, B Aguero, D Quandt, NJ Wickett, AJ Shaw, B Goffinet. 2019. Resolution of the ordinal phylogeny of mosses using targeted exons from organellar and nuclear genomes. Nature Communications 10: 1485. OPEN ACCESS
Villaverde, T, L Pokorny, S Olsson, M Rincón, MG Johnson, EM Gardner, NJ Wickett, J Molero, R Riina, I Sanmartín. 2018. Bridging the micro and macroevolutionary levels in phylogenomics: Hyb-Seq solves relationships from populations to species and above. New Phytologist 220: 636-650. OPEN ACCESS
Guillory, WX, A Onyshchenko, EC Ruck, MB Parks, T Nakov, NJ Wickett, AJ Alverson. 2018. Recurrent loss, horizontal transfer, and the obscure origins of mitochondrial introns in diatoms (Bacillariophyta). Genome Biology and Evolution 6: 1504-1515. OPEN ACCESS
Laricchia, KM, MG Johnson, D Ragone, EW Williams, NJC Zerega, NJ Wickett. 2018. A transcriptome screen for positive selection in domesticated breadfruit and its wild relatives (Artocarpus spp.). American Journal of Botany 105(5): 915-926. OPEN ACCESS
Parks, MB, T Nakov, EC Ruck, NJ Wickett, AJ Alverson. 2018. Phylogenomics reveals an extensive history of genome duplication in diatoms (Bacillariophyta). American Journal of Botany 105(3): 330-347. OPEN ACCESS
Kates, HR, MG Johnson, EM Gardner†, NJC Zerega, NJ Wickett. 2018. Allele phasing has minimal impact on phylogenetic reconstruction from targeted nuclear gene sequences in a case study of Artocarpus. Americal Journal of Botany 105(3): 404-416. OPEN ACCESS
Parks, MB, NJ Wickett, AJ Alverson. 2018. Signal, uncertainty, and conflict in phylogenomic data for a diverse lineage of microbial eukaryotes (diatoms, Bacillariophyta). Molecular Biology and Evolution 35(1): 80-93. OPEN ACCESS
Medina, R, JG Johnson, Y Liu, N Wilding, TA Hedderson, NJ Wickett, B Goffinet. 2018. Evolutionary dynamism in bryophytes: Phylogenomic inferences confirm a rapid radiation in the moss family Funariaceae. Molecular Phylogenetics and Evolution 120: 240-247.
Briscoe, LRE, NJC Zerega, HT Lumbsch, M Stech, E Kraichak, MJ Von Konrat, JJ Engel, NJ Wickett. 2017. Molecular, morphological, and biogeographic perspectives on the classification of Acrobolboideae (Acrobolbaceae, Marchantiophyta). Phytotaxa 319(1): 056-070.
Carvalho-Silva, M, M Stech, LH Soares-Silva, WR Buck, NJ Wickett, Y Liu, PEAS Camara. 2017. A molecular phylogeny of the Sematophyllaceae s.l. (Hypnales) based on plastid, mitochondrial, and nuclear markers, and its taxonomic implications. Taxon 66(4): 811-831.
Johnson, MG, EM Gardner, Y Liu, R Medina, B Goffinet, AJ Shaw, NJC Zerega, NJ Wickett. 2016. HybPiper: Extracting coding sequence and introns for phylogenetics from high-throughput sequencing reads using target enrichment. Applications in Plant Sciences 4(7): 1600016. OPEN ACCESS
Gardner, EM, MG Johnson, D Ragone, NJ Wickett, NJC Zerega. 2016. Low-coverage, whole- genome sequencing of Artocarpus camansi (Moraceae) for phylogenetic marker development and gene discovery. Applications in Plant Sciences 4(7): 1600017. OPEN ACCESS
Johnson, MG, C Malley, B Goffinet, AJ Shaw, NJ Wickett. 2016. A phylotranscriptomic analysis of gene family expansion and evolution in the largest order of pleurocarpous mosses (Hypnales, Bryophyta). Molecular Phylogenetics and Evolution Molecular Phylogenetics and Evolution 98: 29-40.
Honaas, LA, EK Wafula, NJ Wickett, JP Der, Y Zhang, PP Edger, NS Altman, JC Pires, JH Leebens-Mack, CW dePamphilis. 2016. Selecting superior de novo transcriptome assemblies: lessons learned by leveraging the best plant genome. PLoS ONE 11(1): e0146062. OPEN ACCESS
Das, M, M Fernández-Aparicio, Z Yang, K Huang, NJ Wickett, S Alford, EK Wafula, CW dePamphilis, H Bouwmeester, MP Timko, JI Yoder, JH Westwood. 2015. Parasitic plants Striga and Phelipanche dependent upon exogenous strigolactones for germination have retained genes for strigolactone biosynthesis. American Journal of Plant Sciences 6(8): 1151-1166. OPEN ACCESS
Wilson, A, NJ Wickett, P Grabowski, J Fant, J Borevitz, G Mueller. 2015. Examining the efficacy of a genotyping-by-sequencing technique for population genetic analysis of the mushroom Laccaria bicolor with either a reference genome or simple denovo analysis. Mycologia 107(1): 217-226.
Wickett, NJ, S Mirarab, N Nguyen, T Warnow, E Carpenter, N Matasci, S Ayyampalayam, M Barker, JG Burleigh, MA Gitzendanner, BR Ruhfel, E Wafula, JP Der, SW Graham, S Mathews, M Melkonian, DE Soltis, PS Soltis, NW Miles, CJ Rothfels, L Pokorny, AJ Shaw, L DeGironimo, DW Stevenson, B Surek, JC Villarreal, B Roure, H Philippe, CW dePamphilis, T Chen, MK Deyholos, RS Baucom, TM Kutchan, MM Augustin, J Wang, Y Zhang, Z Tian, Z Yan, X Wu, X Sun, G Ka-Shu Wong, J Leebens-Mack. 2014. A phylotranscriptomics analysis of the origin and diversification of land plants. Proceedings of the National Academy of Sciences of the United States of America 111(14): E4859-E4868. OPEN ACCESS
Matasci, N, LH Hung, Z Yan, EJ Carpenter, NJ Wickett, S Mirarab, N Nguyen, T Warnow, S Ayyampalayam, M Barker, JG Burleigh, MA Gitzendanner, E Wafula, JP Der, CW dePamphilis, B Roure, H Philippe, BR Ruhfel, NW Miles, SW Graham, S Mathews, B Surek, M Melkonian, DE Soltis, PS Soltis, C Rothfels, L Pokorny, AJ Shaw, L DeGironimo, DW Stevenson, JC Villarreal, T Chen, TM Kutchan, M Rolf, RS Baucom, MK Deyholos, R Samudrala, Z Tian, X Wu, X Sun, Y Zhang, J Wang, J Leebens-Mack, G Ka-Shu Wong. 2014. Data access for the 1000 Plants (1KP) pilot. Gigascience 3:17. OPEN ACCESS
Wicke, S, KF Müller, CW dePamphilis, D Quandt, NJ Wickett, Y Zhang, SS Renner, GM Schneeweiss. 2013. Mechanisms of Functional and Physical Genome Reduction in Photosynthetic and Nonphotosynthetic Parasitic Plants of the Broomrape Family. The Plant Cell 25(10): 3711-3725. OPEN ACCESS
Villarreal, JC, LL Forrest, NJ Wickett, B Goffinet. 2013. The plastid genome of the hornwort Nothoceros aenigmaticus (Dendrocerotaceae): Phylogenetic signal in inverted repeat expansion, pseudogenization, and intron gain. American Journal of Botany 100(3): 467-477.
Zhang, Y, M Fernandez-Aparicio, EK Wafula, M Das, Y Jiao, NJ Wickett, LA Honaas, PE Ralph, MF Wojciechowski, MP Timko, JI Yoder, JH Westwood, CW dePamphilis. 2013. Evolution of a horizontally acquired legume gene, albumin 1, I the parasitic plant Phelipanche aegyptiaca and related species. BMC Evolutionary Biology 13:48. OPEN ACCESS
Bliss, BJ, S Wanke, A Barakat, S Ayyampalayam, NJ Wickett, PK Wall, Y Jiao, L Landherr, PE Ralph, Y Hu, C Heinhuis, J Leebens-Mack, K Arumuganathan, SW Clifton, SN Maximova, H Ma, CW dePamphilis. 2013. Characterization of the basal angiosperm Aristolochia fimbriata: a potential experimental system for genetic studies. BMC Plant Biology 13:13. OPEN ACCESS
Honaas, LA, EK Wafula, Z Yang, JP Der, NJ Wickett, NS Altman, CG Taylor, JI Yoder, MP Timko, JH Westwood, CW dePamphilis. 2013. Functional genomics of a generalist parasitic plant: Laser microdissection of host-parasite interface reveals host-specific patterns of parasite gene expression. BMC Plant Biology 13:9. OPEN ACCESS
Merckx, VSFT, JV Freudenstein, J Kissling, MJM Christenhusz, RE Stotler, B Crandall-Stotler, NJ Wickett, PJ Rudall, HM de Kamer, PJM Maas. 2013. Taxonomy and Classification In Merckx, V (Ed.) Mycoheterotrophy: The Biology of Plants Living on Fungi. Springer, 356pp.
Fernández-Aparicio, M, K Huang, EK Wafula, LA Honaas, NJ Wickett, MP Timko, CW dePamphilis, JI Yoder, JH Westwood. 2013. Application of qRT-PCR and RNA-Seq analysis for the identification of housekeeping genes useful fo normalization of gene expression values during Striga hermonthica development. Molecular Biology Reports 40(4): 3395-3407.
Jiao, Y, J Leebens-Mack, S Ayyampalayam, JE Bowers, MR McKain, J McNeal, M Rolf, DR Ruzicka, E Wafula, NJ Wickett, X Wu, Y Zhang, J Wang, Y Zhang, EJ Carpenter, MK Deyholos, TM Kutchan, AS Chanderbali, PS Soltis, DW Stevenson, R McCombie, JC Pires, G Wong, DE Soltis, CW dePamphilis. 2012. A genome triplication associated with early diversification of the core eudicots. Genome Biology 13: R3. OPEN ACCESS
McKain, MR, NJ Wickett, Y Zhang, S Ayyampalayam, WR McCombie, MW Chase, JC Pires, CW dePamphilis, J Leebens-Mack. 2012. Phylogenomic analysis of transcriptome data elucidates co-occurrence of a paleopolyploid event and the origin of bimodal karyotypes in Agavoideae (Asparagaceae). American Journal of Botany 99(2): 397-406.
Bandaranayake, PCG, A Tomilov, NB Tomilova, QA Ngo, NJ Wickett, CW dePamphilis, JI Yoder. 2012. The TvPirin gene is necessary for haustorium development in the parasitic plant Triphysaria versicolor. Plant Physiology 158(2): 1046-1053. OPEN ACCESS
Westwood, JW, CW dePamphilis, M Das, M Fernandez-Aparicio, LA Honaas, MP Timko, NJ Wickett, JI Yoder. 2012. The Parasitic Plant Genome Project: New tools for understanding the biology of Orobanche and Striga. Weed Science 60(2): 295-306.
Wickett, NJ, LA Honaas, EK Wafula, M Das, K Huang, B Wu, L Landherr, MP Timko, J Yoder, JH Westwood, CW dePamphilis. 2011. Transcriptomes of the parasitic plant family Orobanchaceae reveal surprising conservation of chlorophyll synthesis. Current Biology 21(24): 2098-2104. OPEN ARCHIVE
Wickett, NJ, LL Forrest, JM Budke, B Shaw & B Goffinet. 2011. Frequent pseudogenization and loss of the plastid-encoded, sulfate transport gene cysA throughout the evolution of liverworts. American Journal of Botany 98(8): 1263-1275.
Hsu CY, JP Adams, H Kim, K No, C Ma, SH Strauss, J Drnevich, L Vandervelde, JD Ellis, BM Rice, NJ Wickett, LE Gunter, GA Tuskan, AM Brunner, GP Page, A Barakat, JE Carlson, CW dePamphilis, DS Luthe & C Yuceer. 2011. FLOWERING LOCUS T Duplication Coordinates Reproductive and Vegetative Growth in Perennial Poplar. Proceedings of the National Academy of Sciences of the United States of America 108(26): 10756-10761.OPEN ACCESS
Jiao Y, NJ Wickett, S Ayyampalayam, A Chanderbali, L Landherr, PE Ralph, LP Tomsho, Y Hu, H Liang, PS Soltis, DE Soltis, SW Clifton, SE Schlarbaum, SC Schuster, H Ma, J Leebens-Mack & CW dePamphilis. 2011. Ancestral polyploidy in seed plants and angiosperms. Nature 473: 97-100.
Forrest LL, NJ Wickett, CJ Cox & B Goffinet. 2011. Deep sequencing of Ptilidium pulcherrimum suggests evolutionary stasis in liverwort chloroplast structure. Plant Ecology and Evolution 144(1): 29-43.
Liang H, S Ayyampalayam, NJ Wickett, A Barakat, Y Xu, L Landherr, P Ralph, T Xu, SE Schlarbaum, H Ma, JH Leebens-Mack & CW dePamphilis. 2011. Generation of a large-scale genomic resource for functional and comparative genomics in Liriodendron tulipifera L. Tree Genetics and Genomes 7(5): 941-954.
Preußing M, S Olsson, A Schäfer-Verwimp, NJ Wickett, S Wicke, D Quandt & M Nebel. 2010. New insights in the evolution of the liverwort family Aneuraceae (Metzgeriales, Marchantiophyta) with an emphasis on the genus Lobatiriccardia. Taxon 59(5): 1424-1440.
Wickett NJ, Y Fan, PO Lewis & B Goffinet. 2008. Distribution and evolution of pseudogenes, gene losses and a gene rearrangement in the plastid genome of the non- photosynthetic liverwort, Aneura mirabilis (Metzgeriales, Jungermanniopsida). Journal of Molecular Evolution 67: 111-122.
Wickett NJ, Y Zhang, SK Hansen, JM Roper, JV Kuehl, SA Plock, PG Wolf, CW dePamphilis, JL Boore & B Goffinet. 2008. Functional gene losses occur with minimal size reduction in the plastid genome of the parasitic liverwort Aneura mirabilis. Molecular Biology and Evolution 25(2): 393-401. FREE ACCESS
Wickett, NJ, & B Goffinet. 2008. Origin and relationships of the myco-heterotrophic liverwort Cryptothallus mirabilis Malmb. (Metzgeriales, Marchantiophyta). Botanical Journal of the Linnean Society 156: 1-12.
Goffinet B, NJ Wickett, O Werner, RM Ros, AJ Shaw & CJ Cox. 2007. Distribution and phylogenetic significance of the 71 kb inversion in the chloroplast genome in the Funariidae (Bryophyta). Annals of Botany 99: 747-753. FREE ACCESS
Goffinet B, AJ Shaw, CJ Cox, NJ Wickett & S Boles. 2004. Phylogenetic inferences in the Orthotrichoideae (Orthotrichaceae: Bryophyta) based on variation in four loci from all genomes. Monographs in Systematic Botany from the Missouri Botanical Garden 98:270-289.
Oenothera section Gaura. Norm's backyard.